Fig. 4: PfCERLI2 localises to the rhoptry bulb. | Communications Biology

Fig. 4: PfCERLI2 localises to the rhoptry bulb.

From: Cell biological analysis reveals an essential role for Pfcerli2 in erythrocyte invasion by malaria parasites

Fig. 4

a 2D Confocal immunofluorescence microscopy of early PfCERLI2HAGlmS schizonts stained with DAPI (nucleus) and anti-HA (PfCERLI2) antibodies, along with antibodies to either RAP1 (rhoptry bulb), RON4 (rhoptry neck), AMA1 (micronemes), or MSP1-19 (merozoite surface). Scale bar = 2 µm. b Quantification of colocalisation between PfCERLI2 and the merozoite organelle markers RAP1, RON4, AMA1 and MSP1-19. Colocalisation quantified as Pearson’s correlation coefficient (PCC) when the PfCERLI2 signal was defined as the region of interest. n = 3 biological replicates, 6 schizonts per replicate. Error bars = SEM, ***p < 0.001, ****p < 0.0001, by unpaired t-test. c Representative maximum-intensity projection of PfCERLI2HAGlmS parasites stained with antibodies to RAP1 (rhoptry bulb) and HA (CERLI2) and imaged using the super-resolution microscopy platform Airyscan. Yellow box indicates the zoom area for the right-hand panel.

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