Fig. 8: DiQ-BioID of PfCERLI1 and PfCERLI2 reveals their protein interactome. | Communications Biology

Fig. 8: DiQ-BioID of PfCERLI1 and PfCERLI2 reveals their protein interactome.

From: Cell biological analysis reveals an essential role for Pfcerli2 in erythrocyte invasion by malaria parasites

Fig. 8

a A schematic of the DiQ-BioID system under control and test conditions. b Pfcerli1DiQ-BioID and Pfcerli2DiQBioID parasites were treated for 8 h on rapalog to permit for dimerisation between the GFP-tagged bait construct and mCherry-tagged BirA, visualised by live fluorescence. Scale bars = 2 μm. c Biotinylation at the rhoptry bulb was confirmed by co-localising foci between RAP1 staining and a streptavidin-conjugated AlexaFluor488 in fixed cells by IFA. Scale bars = 2 μm. d Protein interactors for PfCERLI1. N = 3 biological replicates. e Protein interactors for PfCERLI2. N = 3 biological replicates. d, e Colour code corresponds to: green (bait protein), yellow (peak schizont-expressed), pink (late trophozoite to schizont stage-expressed), blue (uniformly or ring stage-expressed). *P-value calculated by t-test between intensity values in double-treated parasites and no rapalog controls. Proteins not identified in the no rapalog control were assigned with an intensity value of 0.

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