Fig. 1: PDE4D expression is upregulated in hypertensive mice. | Communications Biology

Fig. 1: PDE4D expression is upregulated in hypertensive mice.

From: Phosphodiesterase 4D promotes angiotensin II-induced hypertension in mice via smooth muscle cell contraction

Fig. 1

a Real-time polymerase chain reaction (RT-PCR) was used to measure mRNA expression of PDE4A, PDE4B, PDE4C, and PDE4D in the aorta tissues of control mice and hypertensive mice (fold change vs. one of controls). b Representative western blot showing PDE4D expression in aorta tissues. c Quantification of PDE4D expression normalized to glyceraldehyde-3-phosphate dehydrogenase (GAPDH) protein. d Representative immunohistochemical staining of PDE4D. Arrows indicate positive areas. e Quantification of the percentage of PDE4D-positive area. n = 4 in control group, n = 8 in hypertensive group. Data are expressed as mean ± standard error of mean (SEM). Two-tailed Student’s t test was performed to compare differences between two groups. *p < 0.05, ***p < 0.001. L lumen.

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