Fig. 4: MoDCs remodel iron homoeostasis in response to S. Typhi. | Communications Biology

Fig. 4: MoDCs remodel iron homoeostasis in response to S. Typhi.

From: Dual RNA sequencing reveals dendritic cell reprogramming in response to typhoidal Salmonella invasion

Fig. 4

a Surface expression of TFRC measured by flow cytometry over 24 h in MoDCs infected with STM-LT2, STM-D23580 or ST-Ty2 or left uninfected. Mean ± SEM from four independent experiments are shown. Two-way ANOVA, P value <0.05 (*), <0.01 (**), <0.001 (***). b Surface expression of TFRC measured by flow cytometry on MoDCs stimulated with heat-killed (HK) bacteria for 24 h. Mean ± SEM from four independent experiments are shown. One-way ANOVA test. Flow cytometry analysis of the transferrin– FITC internalisation assay in infected (c) or bystander (d) MoDCs. Mean ± SEM from four independent experiments are shown. Two-way ANOVA, P value <0.05 (*), <0.01 (**), <0.001 (***). MoDCs pre-treated with FeC6H5O7 (e) or the iron chelator Deferoxamine (DFO) (f) were infected with STM-LT2, STM-D23580, ST-Ty2 or ST-CT18. Bacterial CFU was determined at 2 h, 8 h and 24 h p.i. White bars indicate untreated MoDCs used as controls. Mean ± SEM from six independent experiments are shown. Two-way ANOVA, P value <0.05 (*), <0.01 (**), <0.001 (***).

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