Fig. 5: Stress-resistant mechanisms employed by S. Typhi during infection of MoDCs.
a Salmonella surviving after challenge with 0.1 mM hydrogen peroxide (H2O2) for 5, 15 or 60 min. The mean ± SEM from four independent experiments is shown. Two-way ANOVA test, P value <0.05 (*), <0.01 (**). b Box plot displaying the normalised gene expression level of katG (P value <0.01) in intracellular bacteria or liquid culture controls. c Flow cytometry analysis of ROI production in MoDCs infected pre-treated with FeC6H5O7 or the iron chelator DFO and infected for 6 h with Salmonella strains. Mean ± SEM from three independent experiments are shown. Two-way ANOVA, P value <0.01 (**). d Salmonella surviving after challenge with 0.5 mM Spermin NONOate for 5, 15 or 60 min. The mean ± SEM from five independent experiments is shown. Two-way ANOVA test, P value <0.001 (***), <0.0001 (****). e Box plots displaying the normalised gene expression level of hmpA and ygaD (P value <0.01) in intracellular bacteria or liquid culture controls. f Box plots showing gene expression of hmpA detected by qPCR in multiple Salmonella strains after challenging with 0.5 mM Spermin NONOate for 5 min. Mean ± SEM from four independent experiments are shown. Two-way ANOVA test, P value <0.05 (*), <0.01 (**), <0.0001 (****). g MoDCs pre-treated with FeC6H5O7 were infected with STM-LT2, STM-D23580, ST-Ty2 or ST-Ty2 ΔhmpA. Bacterial CFU were determined at 24 h p.i. White bars indicate untreated MoDCs used as controls. Mean ± SEM from five independent experiments are shown. Two-way ANOVA, P value <0.05 (*), <0.01 (**).
