Fig. 6: Ex vivo validation. | Communications Biology

Fig. 6: Ex vivo validation.

From: Dual RNA sequencing reveals dendritic cell reprogramming in response to typhoidal Salmonella invasion

Fig. 6

a Gene expression of FTH1, TFRC and SLC40A1 measured by qPCR at 4 h p.i. in Terminal Ileo biopsies infected with Salmonella strains. Mean ± SEM from four independent experiments are shown. Two-way ANOVA test, P value <0.05 (*), <0.01 (**). b Terminal Ileo biopsies pre-treated with FeC6H5O7 or the iron chelator Deferoxamine (DFO) were infected with STM-LT2, STM-D23580 or ST-Ty2. Bacterial CFU were determined at 4 h p.i. Mean ± SEM from five independent experiments are shown. Two-way ANOVA, P value <0.05 (*), <0.01 (**). c Terminal Ileo biopsies pre-treated with FeC6H5O7 or the iron chelator Deferoxamine (DFO) were infected with STM-LT2, STM-D23580 or ST-Ty2 and IL-1β, IL-12p70 and IFN-γ were quantified by ELISA at 4 h p.i. The mean ± SEM from three independent experiments is shown. Two-way ANOVA test, P value <0.05 (*), <0.01 (**), <0.001(***), <0.0001(****).

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