Fig. 5: The “hot spots” characterized in BsGT-1 also exist in its homologs.
From: Docking-guided rational engineering of a macrolide glycosyltransferase glycodiversifies epothilone B
a The alignment of BsGT-1 with its four homologs YjiC, BaGT, BgGT, and BpGT, wherein the “hot spots” of I62, F231, M296, and Q318 characterized in BsGT-1 were highlighted. b The elevated conversion efficiency towards the sugar donors UDP-GlcNAz (2h), UDP-Gal (2i), and GlcNAc (2e) achieved by the mutations of F231S, M296A, and Q318C, respectively. The displayed values (fold change) are the conversion efficiency of mutants relative to the respective wild type. c Comparison of the conversion rate (mean ± SD) of glycosides 1a–1d by the mutants (I62A or V62A) and wild type (WT). The 62nd site in the five GTs was uniformly related to polysaccharification because the proportion of monoglycoside 1a was reduced and the polyglycosides (1b–1d) in the mutant were accordingly increased, the conversion data were obtained from three independent repeat experiments, the displayed fold changes were calculated based on the averages of the conversion data.
