Fig. 1: Experimental setup to screen small and large intestine-derived monolayers for drug toxicity.
From: Differential toxicity to murine small and large intestinal epithelium induced by oncology drugs
a Workflow for culturing and characterizing the effect of oncology drugs in small and large intestine-derived monolayers. Crypts isolated from harvested murine small and large intestines (white arrowheads) are cultured as intestinal monolayers. Intestinal monolayers are grown in control media for 24 h, followed by drug incubation for 48 h. Total and proliferative cell numbers are measured from images of stained intestinal monolayers. Microscope cartoon was created with BioRender.com. b Tree plot of the drug classes included in the drug panel. n: number of drugs per class. c Images of small and large intestine-derived monolayers grown in control media for 72 h. Scale bars, 20 µm. The total number of cells per well were determined via nuclei segmentation (Hoechst stain) and the number of proliferative cells per well were determined via EdU+ nuclei segmentation. Boxplot showing median value, whiskers showing lower 10th and upper 90th percentiles. n = 72 wells. SI: small intestine; LI: large intestine.
