Fig. 2: Increased expression of MIF gene in TET2-mutant CMML monocytes.

a–c Sequencing of total polyA-RNA was performed in sorted peripheral blood monocytes collected from age-matched healthy donors (n = 10, gray circles) and CMML patients (n = 60) including TET2^WT (n = 17, blue squares) and TET2^MUT (n = 43, orange triangles) cases. a MIF gene expression after count normalization. Data are mean +/− SEM of indicated biological samples. Mann–Whitney test using TET2^WT as control: *P < 0.05. b Inverse correlation between TET2 and MIF gene expression in all samples (10 age-matched healthy donors and 60 CMML patients); R² = 0.2325; P < 0.0001. c RT-qPCR analysis (normalized to PPIA gene) of MIF mRNA expression in sorted peripheral blood monocytes of 19 young healthy donors (age < 65), 8 age-matched healthy donors (age > 64) and 146 CMML patients (TET2^WT 56; TET2^MUT 90). Data are mean +/− SEM of indicated biological samples. Dunnett’s multiple comparison test using Old HD as control, **P < 0.01, ns, non-significant. d Heatmap of the expression of a selection of cytokine and chemokine genes in the three cohorts. e TET2 and MIF gene expression after count normalization was compared in CMML monocytes of patients with truncating tr-TET2^MUT (n = 34) and non-truncating (n = 9) non-trTET2^MUT. Data are mean +/− SEM of indicated biological samples. Dunnett’s multiple comparison test using TET2^WT (n = 17) as control, *P < 0.05, ns, non-significant. f MIF gene expression normalized to PPIA gene was compared in CMML monocytes of patients with truncating trTET2^MUT (n = 68) and non-truncating (n = 22) non-trTET2;^MUT Data are mean +/− SEM of indicated biological samples. Dunnett’s multiple comparison test using TET2^WT (n = 56) as control, ****P < 0.0001, ns, non-significant; g MIF concentrations in bone marrow fluid from healthy controls (N = 10), TET2^WT (n = 12), trTET2^MUT (n = 19) and non-trTET2^MUT (n = 3) CMML patients. Data are mean +/− SEM of indicated biological samples. Dunnett’s multiple comparison test using Old HD as control, **P < 0.01, ns non-significant.