Fig. 3: TET2 binds to the proximal promoter of MIF.
From: Macrophage migration inhibitory factor is overproduced through EGR1 in TET2low resting monocytes
a Schematic representation of MIF gene promoter. Quantitative ChIP-PCR analyses were performed in kasumi-1 cells infected by SCR (yellow) or TET2 (blue) shRNAs by using a set of primers targeting regions located in distal (R1), intermediate (R2), proximal (R3) promoter, and in exon 1, closed to the TSS (R4). b TET2-associated DNA was immunoprecipitated with either C2 (TET2 Ab1 upper panel) or sc-136926 (TET2 Ab2, lower panel) antibodies. c H3K4me3 (upper panel) or H3K27me3 (lower panel) marks associated with DNA immunoprecipitation. d RNA polymerase II phosphorylated on CTD serine 5 (pS5 Pol II) associated to DNA immunoprecipitation. b–d Results are expressed as fold enrichment relative to DNA immunoprecipitated with control immunoglobin G (IgG). Data are mean +/− SEM of each sample run in triplicate. Unpaired t Test, *P < 0.05.
