Fig. 4: Identification of a minimal proximal promoter of MIF gene.
From: Macrophage migration inhibitory factor is overproduced through EGR1 in TET2low resting monocytes
a Kasumi-1 cells stably transduced with SCR and TET2 shRNAs were transiently transfected with the pGL3 vector encoding the Luciferase gene alone (LUC) or LUC gene under control of indicated MIF promoter fragments. Cells were co-transfected with a Renilla luciferase construct for normalization and results are the ratio between luciferase and Renilla activities. Data are mean +/− SEM from at least six independent well experiments; Unpaired Students-t test, *P < 0.05. b The same experiments were performed using the −157/+129 MIF promoter sequence in which mutations were induced in the DNA binding sites c-Myb, CREd, CREp, SP1d, SP1p, AML1a, AP4, and HIF1. Data are mean +/− SEM from seven independent well experiments; Unpaired t test, *P < 0.05. c DNA consensus sequences for SP1d, SP1p, and EGR transcription factors in MIF proximal promoter.
