Fig. 6: EGR1 recruitment to DNA is modified by TET2 mutation in monocytes.

ChIP-seq experiments were performed using an anti-EGR1 antibody in sorted peripheral blood monocytes from 2 healthy donors (control 1 and 2) and three CMML patients (CMML1268: TET2 H800fs, VAF = 16%; CMML1818: TET2 A1241fsX1, VAF = 40% and TET2 E1513GfsX9, VAF = 49%; CMML1900: TET2 W564X, VAF = 100%). a Peak calling for EGR1 on MIF gene in controls (pink) and CMML (dark blue). b Repartition of EGR1 peaks on the genome compared to hg19 reference annotation (genome). c Ranking heatmaps of EGR1 peaks centered on gene transcription starting sites (TSS). d Peak calling for EGR1 in control (pink) and CMML (dark blue) monocytes on RAD50 and CEBPD genes. e Venn diagram of peak calling in two healthy donors and f two CMML samples with high VAF trTET2^MUT. g Venn diagram of peak calling comparing two controls and two CMML samples with high VAF trTET2^MUT or h with the CMML sample with low VAF trTET2^MUT.