Fig. 7: MIF promotes monocyte differentiation.

a CD34⁺ cells were sorted from the peripheral blood of healthy donor (n = 5) and CMML patients, without (n = 13) or with TET2 (n = 9) mutation, and analyzed by Agilent microarrays. Indicated gene expression was monitored (Log2 intensity). Data are mean +/− SEM of indicated biological samples. Dunnett’s multiple comparison test using healthy donor as control, *P < 0.05, ns, non-significant. b Inverse correlation between SPI1 and GFI1 Log2 intensity expression in CD34⁺ samples. R² = 0.24; p = 0.02. c Monocytes to polymorphonuclear cell ratio in the peripheral blood of CMML patients without (n = 13) or with TET2 (n = 9) mutation. Mann–Whitney test: **P < 0.005. d Cord blood CD34⁺ cells were induced to differentiate as described in Fig. 1, in the presence or the absence of 20 ng/mL MIF for 5–9 days before measuring the fraction of CD14⁺ cells by flow cytometry. Data are mean +/− SEM from eight independent experiments; Mann–Whitney test: **P < 0.005. e Single cell analysis of cord blood CD34⁺ cells induced to differentiate as described in Fig. 1 with or without MIF at day 7. Umaps of cluster analysis at 17 dimensions with a resolution of 0.2. f Umaps of CSF1R and GFI1 gene expressions in monocyte and granulocyte clusters in CTRL and MIF conditions. Scales indicate the intensity of expression. g Percentage of monocytes and granulocytes in the myeloid compartment in CTRL and MIF conditions.