Table 1 Expected and measured masses of DNA fragments after processing of F26-seq1 by drUvrABC.
Oligonucleotide | Sequence | Expected mass (Da) | Measured mass (Da) |
|---|---|---|---|
5′-ATTO633-F26-seq1 (substrate) | 5′- XGAC TAC GTA CTG TTA CGG CTC CAT CFdTC TAC CGC AAT CAG GCC AGA TCT GC -3′ | 16,465.0 | 16,457.5 |
Rev-seq1 (substrate) | 5′- GCA GAT CTG GCC TGA TTG CGG TAG AGA TGG AGC CGT AAC AGT ACG TAG TC -3′ | 15,531.0 | 15,529.7 |
12mer (product) | 5′- pCTCCATCFdTCTAC -3′ | 4,107.4 | 4,109.1 |
18mer (product) | 5′- XGACTACGTACTGTTACGG -3′ | 6,226.6 | 6,226.5 |
20mer (product) | 5′- pCGCAATCAGGCCAGATCTGC -3′ | 6,167.0 | 6,165.4 |
