Fig. 2: Plaque quantification and characterization in the lesser curvature of the aortic arch.
From: Macrophage Jak2 deficiency accelerates atherosclerosis through defects in cholesterol efflux
a, b Representative images of the lesser curvature of the aortic arch sagittal sections stained with H&E from M-Jak2 KO and WT mice fed 16 weeks of HCD starting at 6 weeks of age. Scale bar, 200 µm. c Quantification of lesion size at the lesser curvature (mm2) (n = 14–18/genotype). d, e Representative images of the lesser curvature immunostained with anti-Mac3. Scale bar, 100 μm. f Quantification of Mac3 immunostained area expressed as a percentage of total plaque area (n = 13–14/genotype). g, h Representative images of the lesser curvature immunostained with anti-α-smooth muscle actin (SMA). Scale bar, 100 μm. i Quantification of α-SMA immunostained area expressed as a percentage of total plaque area (n = 13/genotype). j, k Representative images of the lesser curvature from aortic arch sections combined stained with Masson trichrome and Verhoeff Van Giesen. Scale bars, 100 μm. l Quantification of collagen expressed as a percentage of total plaque area using Masson trichrome stain (n = 7/genotype). Statistical analysis: two-tailed unpaired t tests were performed. Data are presented as the mean ± SEM, *P < 0.05, **P < 0.01, ***P < 0.001. See also Fig. 1 and Supplementary Fig. S4.
