Fig. 3: Decreased cholesterol efflux in Jak2-deficient macrophages.
From: Macrophage Jak2 deficiency accelerates atherosclerosis through defects in cholesterol efflux
a Radioactive cholesterol uptake in BMDM of M-Jak2 KO and WT mice (n = 4–6/genotype). b Quantification of the number of lipid droplets sized 0.3 µm per field stained with BODIPY, in BMDM untreated or treated with LDL, oxLDL, or combined oxLDL and ApoA1 for 24 h (n = 4–8/genotype). c–j Representative images of BMDM in the conditions described in (b). Scale bar, 6.6 µm. k, l Cholesterol efflux measurements using radioactive cholesterol from BMDM; total unstimulated, in response to ApoB-depleted plasma (n = 9/group) and to ApoA1 (n = 8/group). m, n BMDM stimulated with cAMP to induce ABCA1, and total efflux from M-Jak2 KO and WT BMDM in response to ApoB-depleted plasma (n = 9/group) and to ApoA1 (n = 8/group). o–r Cholesterol efflux measurements after JAK2 inhibition with AG490 from M-Jak2 KO and WT BMDM (n = 8/group). Statistical analysis: a, b Multiple t tests with Holm–Sidak correction, k–n two-tailed unpaired t tests, and (o–r) two-way ANOVA with Tukey’s multiple comparisons were performed. Data are presented as the mean ± SEM, *P < 0.05, **P < 0.01, ***P < 0.001, ****P < 0.0001. See also Supplementary Figs. S10.
