Fig. 3: Permanent tumorous mechanical stresses over-stimulate Lgr5+ SC number and CD133 CSC production, in vivo. | Communications Biology

Fig. 3: Permanent tumorous mechanical stresses over-stimulate Lgr5+ SC number and CD133 CSC production, in vivo.

From: Ret kinase-mediated mechanical induction of colon stem cells by tumor growth pressure stimulates cancer progression in vivo

Fig. 3

a Lgr5+ SC in Lgr5-EGFP mice after application of a permanent magnetic compression mimicking tumor growth pressure for 1 month. Control: mice without UML injection (n = 7 mice); UML: mice injected with UML without magnet implantation (n = 8 mice); UML Magnet: mice injected with UML plus permanent magnet implantation for 1 month (n = 6 mice). Scale bar is 10 μm. b Lgr5+ SC in Apc;Lgr5-EGFP mice after application of a permanent magnetic compression mimicking tumor growth pressure for 1 month. Control: mice without UML injection (n = 5 mice); UML: mice injected with UML without magnet implantation (n = 2 mice); UML Magnet: mice injected with UML plus permanent magnet implantation for 1 month (n = 5 mice). Scale bar is 10 μm. c Quantification of a. Mean number of Lgr5-EGFP + SC per crypt and per mouse. Mann–Whitney test; **p < 0.01. d Quantitative analysis of b. Mean number of Lgr5-EGFP + SC per crypt and per mouse. Mann–Whitney test: **p < 0.01; ns not significant. e CD133 + CSC after application of a permanent magnetic compression mimicking tumor growth pressure for 1 month in Apc;Lgr5-EGFP mice. Controls: 4 and 9 month-old mice (n = 5 mice); Control 5 month-old mice having gastric tumors: small intestine and colon (n = 3 mice); UML Magnet 1 m: mice injected with UML plus permanent magnet implantation for 1 month (n = 5 mice). Red arrows show CD133 + crypts. Small white frames show similar images with Lgr5-EGFP and nuclei staining. Scale bar is 10 μm. f Quantitative analysis of e. Percentage of CD133 + crypts per mouse. Mann–Whitney test; *p < 0.05. Error bars: standard deviation.

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