Fig. 4: The mechanical stimulation of SC and CSC multiplication is pRet dependent in Apc heterozygous mice colon, in vivo. | Communications Biology

Fig. 4: The mechanical stimulation of SC and CSC multiplication is pRet dependent in Apc heterozygous mice colon, in vivo.

From: Ret kinase-mediated mechanical induction of colon stem cells by tumor growth pressure stimulates cancer progression in vivo

Fig. 4

a Levels of pRet+ crypts (up), Lgr5+ SC (middle), and CD133 + CSC (down) in Apc;Lgr5-EGFP mice after application of a permanent magnetic compression mimicking tumor growth pressure for 1 month, with and without Vande. Control Veh: mice without UML injection treated for 1 month with vehicle of Vande (n = 8 mice); UML + Magnet 1 m Veh: mice injected with UML plus permanent magnet implantation and treated with vehicle for 1 month (n = 9 mice); Control Vande: mice without UML injection treated for 1 month with Vande (n = 6 mice); UML + Magnet 1 m Vande: mice injected with UML plus permanent magnet implantation and treated with Vande for 1 month (n = 8 mice). N = 2 experiments. Red arrows show CD133 + stained cells. Small white frames show similar images with Lgr5-EGFP and nuclei stainings. Scale bar is 10 μm. b Quantitative analysis of the pRet signal. Percentage of crypts with ≥4 pRet positive cells per mouse. Mann–Whitney test; **p < 0.01 and ****p < 0.0001. c Quantitative analysis of Lgr5-EGFP signal. Mean number of Lgr5+ SC per crypt and per mouse. Mann–Whitney test two-tailed; **p < 0.01. d Quantitative analysis of CD133 signal. Percentage of CD133 + crypts per mouse. Mann–Whitney test; ***p < 0.001. e CD133 + cancer cell marker in Apc and Apc;N1Cre-ERT2 old mice (16 month-old). CD133 antibody staining in colon crypts of mice treated with vehicle (n = 6 mice) and Vande (n = 7 mice). Red arrows show CD133 + stained cells. Scale bar is 10 μm. (f), Quantification analysis of e. Percentage of CD133 + crypts per mouse. Mann–Whitney test. *p<0.05. Error bars: standard deviation.

Back to article page