Fig. 5: Paneth niche cells of the intestine are mechanically induced in Apc heterozygous mice colon in a Ret mechanosensitive dependent process. | Communications Biology

Fig. 5: Paneth niche cells of the intestine are mechanically induced in Apc heterozygous mice colon in a Ret mechanosensitive dependent process.

From: Ret kinase-mediated mechanical induction of colon stem cells by tumor growth pressure stimulates cancer progression in vivo

Fig. 5

a Lysozyme staining of the colon tissue after application of a permanent magnetic compression mimicking tumor growth pressure for 1 month (n = 8 mice) and 3.5 months (n = 4 mice), compared to control (n = 8 mice) in Apc mice. Small boxes display lysozyme positive signal in red to increase contrast with DAPI in blue. Scale bar is 10 μm. b Lysozyme staining of the colon tissue after application of a permanent magnetic compression mimicking tumor growth pressure for 1 month, treated with the pRet inhibitor Vande in Apc;Lgr5-EGFP mice. Control Veh: mice treated with the vehicle of Vande (n = 8 mice); UML Magnet 1 m Veh: mice injected with UML plus permanent magnet implantation for 1 month and vehicle treated (n = 8 mice); Control Vande: mice treated with Vande for 1 month (n = 6 mice); UML Magnet 1 m Vande: mice injected with UML plus permanent magnet implantation for 1 month and Vande treated (n = 9 mice). Small boxes display lysozyme positive signal in red to increase contrast with DAPI in blue. c Quantification of a. Mean number of lysozyme positive crypts per mice. Mann–Whitney test; *p < 0.05; **p < 0.01. d Quantification of b. Mean number of lysozyme positive crypts per mice. Mann–Whitney test; **p < 0.01; ns not significant. Error bars: standard deviation.

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