Fig. 6: The pharmacological inhibition of the Ret/β-cat pathway with Vande inhibits mechanical induction of, and spontaneous tumorigenesis initiation in vivo.
a Live imaging of ACFs (orange narrow) at different times of the experiment subjected to the application of a permanent magnetic compression mimicking tumor growth pressure for 1 month, with or without Vande treatment in Apc;Lgr5-EGFP mice. b ACFs number counting at different times until 28 days of the application of permanent magnetic compression mimicking tumor growth pressure application on the colon of Apc;Lgr5-EGFP mice with or without Vande treatment, n = 6–9 mice/condition. N = 2 experiments. Statistical significance determined using the Holm-Sidak method, with alpha = 0.05. Adjusted p (D14, UMLm veh vs. veh) <0.05, adjusted p (D28, UMLm veh vs. veh) <0.01, adjusted p (D14, UMLm vande vs. UMLm veh) <0.05, adjusted p (D28, UMLm vande vs. UMLm veh) <0.01. c Live imaging of ACF (orange arrows) at 28th day of vehicle or Vande treatment of Apc old mice. d ACF number counting at different times during the 1 month of vehicle or Vande treatment, n = 13–14 mice/condition. N = 3 experiments. Statistical significance determined using the Holm-Sidak method, with alpha = 0.05. Adjusted p (D28) <0.01. e Intestine upper section pictures after 1 month of vehicle or Vande treatment. Intestine tumors are surrounded by a red circle. f Quantification of e. N = 7 mice/condition. Statistical significance determined using Mann–Whitney test. Error bars: standard deviation, except for d, f in which it is standard error to the mean.
