Fig. 1: Produce magnetized RBC (mRBC) in vitro.
From: Precise control of embolic stroke with magnetized red blood cells in mice
a-a-i Schematic illustration of the principle of streptavidin-biotin-based mRBC generation. a-ii TEM image and particle diameter analysis a-iii of streptavidin-MNPs synthesized by our collaborators. Representative SEM (b, c) and TEM (d, e) images of unmagnetized RBCs (b, d) and mRBCs (c, e), respectively. Magenta arrows indicate MNPs. f TEM image of ultrathin sections from a segment of the middle cerebral artery, in which mRBCs accumulated. Endothelia and vascular smooth muscle cells are highlighted in magenta and green. Magenta arrows indicate MNPs. Time-lapse pictures of MNP (g), mRBC (g-i) and unmagnetized RBC (g-ii) enrichment upon magnetic gradient. Black arrows indicate aggregated MNPs, and green arrows show accumulated mRBCs. h Quantitative analysis of the RBC magnetization efficiency (SEM, 31.3%, 148 of 427 cells; TEM, 30.1%, 196 of 654 cells, three independent experiments were performed for each group of ‘mRBC enrichment’). The effect size between two groups at doses of 0.5 and 1.5 pg per RBC is 18.2. i Snapshot of trapped DiO-mRBCs on a cylindrical magnet with a 1.5-mm diameter. The dashed circle indicates the edge of the magnet, and white stars indicate the floating unmagnetized DiO-RBCs and DiO-mRBCs. j Still images of the trajectory of one mRBC movement indicated with dashed magenta arrow.
