Fig. 2: mRBCs occlude the targeted microvessels in vivo.
From: Precise control of embolic stroke with magnetized red blood cells in mice
a Schematic illustration of the principle of SIMPLeR by using a magnet to trap mRBCs at the distal MCA. Low- and high-magnification images of unmagnetized DiO-RBCs (b) and DiO-mRBCs (c) upon magnetic gradient. Both unmagnetized DiO-RBCs and DiO-mRBCs were withdrawn 5 min after they were injected into two adult mice. The magenta dashed line indicates the edge of a cylindrical magnet underneath. Magnet arrows indicate the accumulated mRBCs. Three independent experimental repeats were conducted. d Time-lapse fluorescent stereoscopic images of P3 mouse live brains, in which the process of gradual accumulation of DiO-mRBCs (magenta arrows) around the micromagnet (1 mm diameter) was recorded. e Still images of the dispersal process of mRBCs after the magnet was removed. Magenta arrows indicate the dispersing mRBCs. f, g Bright field (f) and fluorescent stereoscopic images of a P3 mouse brain, of which dMCA was occluded by Alexa Fluor 488-mRBCs (magenta arrows). Green arrows indicate the untargeted neighboring vessels. The segment of dMCA in the magenta box was subjected to TEM, as shown in Fig. 3n–o and Supplementary Fig. 5. The illustrations of mouse pup body and brain in (a) were copied from Biorender.com.
