Fig. 4: Both SIMPLeR and SIMPLE resulted in hemorrhagic transformation in neonatal mice.
From: Precise control of embolic stroke with magnetized red blood cells in mice
a–c Macroscopic pictures of brains from P3-5 mouse pups that underwent 6-hour SIMPLeR or SIMPLE at 6 or 80 mg/kg MNPs. d Picture of a brain from embryonic day 19 mice that underwent in utero photothrombosis at E18 taken by dissecting microscopy. e Representative bright-field images of brains of P2-7 mouse pups that underwent different occlusion durations ranging from 2 h to 6 h. f Quantification of the hemorrhagic transformation rate after different occlusion durations. Gray indicates the mild HI type, while black indicates the more severe PH type of hemorrhagic transformation. N = 17 to 41 mouse pups for each group. g Representative pictures of mouse brain and brain sections from P4, P15, and P80 mice that underwent 6–10 h of SIMPLE (P4 and P15) or MCA occlusion(P80). h Quantification of the hemorrhagic transformation rate for g. i Confocal images of the CNS macrophage marker Iba1 in brain sections from vehicle- or PLX 5622-treated mouse pups. j Macroscopic picture of a brain from a P3 mouse pup that had long-term PLX 5622-treatment prior to 6 h of ischemic stroke. k The reverse correlation of CNS macrophage density in the brain cortex and hemorrhagic transformation rate at 6 h of ischemic occlusion. The magenta dashed line represents the trendline. Data are presented as Mean ± SEM.
