Fig. 4: Decreased p300 and Brd3 chromatin binding in DKO MEFs.

a Equal P300 expression in WT and DKO MEFs. b Box plots showing decrease P300 chromatin occupancy at enhancers and promoters of DKO cells. c MA plots showing differences in P300 chromatin binding between DKO and WT cells. Sites showing statistically significant differences (FDR < 0.05) are in red. Blue dots and blue density cloud represents all points corresponding to the non-changing regions. Note that most altered sites show decrease binding in DKO cells. d Profile plots showing decreased P300 occupancy at promoters and enhancers of DKO cells. e Equal Brd3 expression in WT and DKO MEFs. f Box plots showing decrease Brd3 chromatin occupancy at enhancer and promoters of DKO MEFs. g MA plot showing differences in Brd3 chromatin binding between DKO and WT cells. Sites showing significant differences (FDR < 0.05) are in red. Blue dots and blue density cloud represents all points corresponding to the non-changing regions. h Profile plots showing decreased Brd3 occupancy at promoters and enhancers of DKO cells. i IGV tracks showing reduced H3K27ac levels, and reduced P300 or Brd3 chromatin occupancy in DKO cells. All ChIP analyses from two biological replicates.