Fig. 3: Overactivation of NCC during the active period inhibits sodium excretion under NO suppression. | Communications Biology

Fig. 3: Overactivation of NCC during the active period inhibits sodium excretion under NO suppression.

From: Dietary salt with nitric oxide deficiency induces nocturnal polyuria in mice via hyperactivation of intrarenal angiotensin II-SPAK-NCC pathway

Fig. 3

a Urinary sodium excretion per day (left) during active (middle) and inactive (right) periods (NSD, HSD, L-NAME: n = 7, l-NAME + HSD: n = 9). Statistical analysis was performed using the two-way ANOVA or the two-tailed Student’s t-test. b Representative immunoblotting (above) and quantitative analysis (below) of renal phosphorylated NCC (pT53), NCC, ENaCα, ENaCβ, ENaCγ, and ACTN in the active phase for the four groups. Data are expressed as the mean ± SEM. *p < 0.05 (n = 6 mice per group). Statistical analysis was performed using two-way ANOVA. c Representative fluorescent immunostaining of renal phosphorylated NCC (pT53) and total NCC for the four groups using serial sections. Original magnification: ×200. Scale bar, 100 μm. All data are expressed as the mean ± SEM, *p < 0.05.

Back to article page