Fig. 2: Both BLI and SPR show the specific interaction between fortilin and TGF-β1.
From: Fortilin interacts with TGF-β1 and prevents TGF-β receptor activation
BLI, bio-layer interferometry; Kd, dissociation constant; SPR, surface plasmon resonance; His6-fortilin, hexa-his-tagged recombinant fortilin; TGFβRIIFc, the ligand-binding segment of the TGF-β receptor II conjugated to the Fc portion of the IgG. a BLI showed the specific binding of fortilin to TGF-β1 at Kd of 94.5 nM. b SPR showed that fortilin binds TGF-β1 at Kd of 210.5 nM. c After fortilin was conjugated to the SPR chip surface, TGFβRIIFc mixed with various concentrations of TGF-β1 was injected onto the chip surface to evaluate the binding of fortilin to TGF-β1 in the presence of TGFβRII. There was dose-dependent disruption by TGFβRII of the fortilin-TGF-β1 interaction. Because fortilin did not bind TGFβRIIFc (Fig. S2b), the data suggest that fortilin and TGFβRII competitively bind TGF-β1.
