Fig. 5: Fortilin inhibits TGF-β1-induced differentiation of the C3H10T1/2 mesenchymal progenitor cells.
From: Fortilin interacts with TGF-β1 and prevents TGF-β receptor activation
CNN1, Calponin 1; α-SMA, alpha-smooth muscle actin; SMA22α, smooth muscle protein 22α; A.U. arbitrary unit; Data points, means ± SD; N, the number of biological replicates; *P < 0.05, ***P < 0.005; ****P < 0.001. C3H10T1/2 cells were starved with DMEM containing 0.1% fetal bovine serum for 48 h followed by incubation with fortilin (0.5 µg/mL), TGF-β1 (1 ng/mL), or their mixture for 8 h. Untreated cells were used as the negative control. Quantitative real-time PCR was performed (N = 3) to determine mRNA expression of CNN1 (a), α-SMA (b), and SMA22α (c) in C3H10T1/2 cells. CYP was used the internal reference. Fortilin inhibited TGF-β1-induced expression of the smooth muscle cell differentiation marker proteins (CNN1, α-SMA, and SMA22α) in C3H10T1/2 cells.
