Fig. 4: OTUD6A binds with Brg1 and AR separately.
From: OTUD6A promotes prostate tumorigenesis via deubiquitinating Brg1 and AR
a The overlapping OTUD6A-interacting partners identified by IP-MS in VCaP and PC-3 cells were clustered through Gene Ontology (GO) analysis (http://david.abcc.ncifcrf.gov). b Co-IP experiments in VCaP cells were performed using anti-OTUD6A (upper) or anti-Brg1, anti-AR (lower) antibodies. IgG was used as a negative control. c, d IB analysis of WCLs and IPs derived from 293T cells transfected with HA-Brg1 (c) or HA-AR (d) together with the indicated constructs of Flag-tagged OTU family members. e IB analysis of the GST pull-down assay. 6A-N, OTUD6A-N-terminus; 6A-C, OTUD6A-C-terminus; 6A-FL, OTUD6A-full length. f, g IB analysis of WCLs and IPs derived from 293T cells transfected with Flag-OTUD6A together with the indicated HA-tagged fragments of Brg1 (f) or HA-tagged fragments of AR (g).
