Fig. 5: OTUD6A stabilizes Brg1 and AR.
From: OTUD6A promotes prostate tumorigenesis via deubiquitinating Brg1 and AR
a IB analysis of VCaP cells infected with the indicated lentivirus for 48 h. b IB analysis of VCaP and 293T cells transfected with the indicated plasmids for 48 hr. c VCaP-shBrg1 cells were infected with the indicated shRNA lentivirus or transfected with the indicated plasmids for 48 h and then harvested for IB analysis. d VCaP and VCaP-shOTUD6A cells were treated with 100 μg/mL CHX for indicated time points and then harvested for IB analysis. Band intensities were quantified by ImageJ software and were normalized using Tubulin intensities. shScr: shScramble; sh6A: shOTUD6A. e IB analysis of WCLs and IPs derived from VCaP cells transfected with the indicated shRNAs and plasmids for 48 h. Cells were treated with 5 μM MG132 for 12 h before harvest. f IB analysis of WCLs and IPs derived from VCaP-shBrg1 cells transfected with the indicated shRNAs and plasmids for 48 h. g IB analysis of WCLs and IPs derived from 293T cells transfected with Flag-OTUD6A or Flag-OTUD6B together with the indicated HA-tagged Brg1 or HA-tagged AR and His-Ub plasmids. h IB analysis of WCLs and IPs derived from 293T cells transfected with the indicated OTUD6A constructs together with HA-Brg1 or HA-AR and His-Ub plasmids. i IB analysis in VCaP cells after transfection with the indicated plasmids for 48 h.
