Fig. 1: In vitro cleavage activities of CjCas9 and enCjCas9.
From: Engineered Campylobacter jejuni Cas9 variant with enhanced activity and broader targeting range
(a–d) In vitro cleavage activities of CjCas9 (a, b) and enCjCas9 (c, d) toward DNA targets with different PAMs. The linearized plasmid targets were incubated with the CjCas9–sgRNA complex at 37 °C for 2 min. Data are mean ± s.d. (n = 3). (e, f) Sequence logos of CjCas9 (e) and enCjCas9 (f) obtained from the in vitro PAM discovery assay. (g, h) 2D PAM profiles of CjCas9 (g) and enCjCas9 (h) obtained from the in vitro PAM discovery assay.
