Fig. 4: Cell surface receptor nanotopography visualized by 3D TRABI imaging.
From: Approach to map nanotopography of cell surface receptors
a Schematic illustration of sample preparation for a receptor nanotopography imaging using dTRABI. A cell in suspension is first immobilized on a glycine-coated coverslip, fixed and imaged using dTRABI approach. The resulting three-dimensional dTRABI image represents a footprint of a cell on the optical surface with the color-coded axial position of localizations (right panel). b Three-dimensional dTRABI image of T-cell surface receptor CD45 with selected ROIs exhibiting a broad z-distribution of receptor localizations. CD45 was labeled with Alexa Fluor 647-conjugated primary antibody (MEM-28). c Magnified x–y and x–z projections of ROIs 1–5 as in b. Blue arrowheads point to microscopic membrane protrusions at the cell edges, red arrowheads to folded nanoscopic protrusions under the cell body and green arrowhead to membrane invagination. Scale bars, 5 μm in b and 500 nm in c.
