Table 1 Primary and secondary antibodies and reagents.
Protein targeted | Host | Dilution | Provider | Catalogue and lot number |
|---|---|---|---|---|
Primary antibodies | ||||
BLT1 | Rabbit | 1:400 | Cayman | 120114; 0495946-1 |
ChemR23 | Mouse | 1:500 | Santa Cruz | SC-398769; J1216 |
FPR2 | Rabbit | 1:500 | Santa Cruz | Sc-66901, C2311 (M-73) |
GABAAR1α | Rabbit | 1:500 | Abcam | Ab252430 |
GFAP | Rabbit | 1:800 | Dako | Z033401-2; 55769 |
GLUR1 | Rabbit | 1:400 | Abcam | Ab31232; GR3379169-1 |
GLUR4 | Rabbit | 1:400 | Merck | AB1508; 3551424 |
GPR18 | Rabbit | 1:400 | Sigma-Aldrich | SAB4501252 |
Iba1 | Rabbit | 1:400 | Wako/Fujifilm | 019-19741; CAK 1997 |
LGR6 | Rabbit | 1:500 | Invitrogen | PA5-102099; VA2931943 |
PSD95 | Mouse | 1:500 | Abcam | Ab2723; GR3248435-12 |
Trem-2 | Sheep | 1:400 | R&D Systems | AF1729; JPN0219101 |
Aβ(1-16) peptide | Mouse | 1:500 | BioLegend | 803001; B291304 |
Secondary antibodies and reagents | ||||
Alexa Fluor Plus 594 anti-mouse | Donkey | 1:400 | Invitrogen | A32744 |
Alexa Fluor Plus 488 anti-rabbit | Donkey | 1:400 | Invitrogen | A32790 |
IRDye®800CW anti-rabbit | Donkey | 1:15 000 | LiCor | 926-32213 |
IRDye®680RD anti-mouse | Donkey | 1:15 000 | LiCor | 926-68072 |
IRDye®800CW anti-goat | Donkey | 1:15 000 | LiCor | 926-32214 |
Intercept® TBS Blocking buffer | LiCor | 927-66003 | ||
