Fig. 4: Full-length MS2 genome replication in PURExpress. | Communications Biology

Fig. 4: Full-length MS2 genome replication in PURExpress.

From: In vitro characterisation of the MS2 RNA polymerase complex reveals host factors that modulate emesviral replicase activity

Fig. 4

a Replication scheme of [F30-Bro(+/−)]MS2(+/−) RNA by the MS2rep complex. b Fluorescence change over the 6-h incubation at 37 °C for replication analysis of 15 nM [F30-Bro(+)]MS2(+) in PURExpress with (blue) and without (red) 1 µM MS2rep. Grey lines indicate average fluorescence levels of reference concentrations of in vitro transcribed [F30-Bro(+)]MS2(+) in PURExpress. Standard deviations are derived from three independent replicates.

Back to article page