Fig. 3: PDGF-BB treatment causes a transient loss of PDGFRβ expression due to internalisation in pericytes. | Communications Biology

Fig. 3: PDGF-BB treatment causes a transient loss of PDGFRβ expression due to internalisation in pericytes.

From: Characterisation of PDGF-BB:PDGFRβ signalling pathways in human brain pericytes: evidence of disruption in Alzheimer’s disease

Fig. 3

Brain pericytes were treated with PDGF-BB (10 ng/mL) or vehicle for the specified time, then stained for cell-surface PDGFRβ for flow cytometry, fixed and stained for total PDGFRβ expression or RNA extracted for qPCR. a Representative images and quantification of b total PDGFRβ immunostaining and c PDGFRβ puncta formation in pericytes treated with vehicle or PDGF-BB. Scale bar = 500 μm, inset = 25 μm. n = 3–4, two-way ANOVA. d Gene expression of PDGFRB in pericytes incubated for different lengths of time with PDGF-BB. Expression of e cell surface and f total PDGFRβ and g the distribution of PDGFRβ determined by flow cytometry. n = 3, one-way ANOVA. Representative histograms of h cell surface and i total PDGFRβ and j representative images of total PDGFRβ expression in cells analysed by flow cytometry. Scale bar = 5 μm. *p < 0.05, **p < 0.01, ***p < 0.001 vs vehicle control, #p < 0.05, ##p < 0.01, ###p < 0.001 vs PDGF-BB-treated.

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