Fig. 1: Identification of SINEs as CRISPR-Cas9 inhibitors. | Communications Biology

Fig. 1: Identification of SINEs as CRISPR-Cas9 inhibitors.

From: KPT330 improves Cas9 precision genome- and base-editing by selectively regulating mRNA nuclear export

Fig. 1

a Schematic presentation of EGFP reporter cell-based screening of CRISPR-Cas9 inhibitors. b Flowchart of the screening process. X, electron withdrawing group. c Potency of SINEs on inhibiting CRISPR-activated EGFP fluorescence. d SINEs at 0.5 μM inhibit the on-target editing activity of CRISPR-Cas9 at HBB and RUNX1 genes in HEK293 cells. The data are shown as mean ± SD (n = 3 biologically independent samples).

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