Fig. 1: HIF1α is upregulated in PFKFB3βKO DS mice under high diabetogenic stress.

a Representative immunofluorescence images of islets from WT, PFKFB3WT DS, and PFKFB3βKO DS mice at 13 weeks HFD immunostained for PFKFB3 (red), insulin (green) and nuclei (blue). b Quantification of images in (a) (**p = 0.0015, ***p = 0.0006). c Representative immunofluorescence images of islets from WT, PFKFB3WT DS and PFKFB3βKO DS mice at 13 weeks HFD immunostained for HIF1α (red), insulin (green) and nuclei (blue). d Quantification of images in (c) (n = 3, n = 4 for PFKFB3βKO DS–independent animals, SEM *p = 0.034).