Fig. 7: PFKFB3^βKO DS mice show a decrease of double- insulin and glucagon positive cells at lower and higher diabetogenic stress.

a Quantification of the ratio between bihormonal (insulin- and glucagon positive) cells relative to all α-, β- and bihormonal cells (%) at 13 weeks of HFD. b Quantification of the ratio between α-cells relative to all α-, β- and bihormonal cells (%) at 13 weeks of HFD. c Quantification of the ratio between β-cells relative to all α-, β- and bihormonal cells (%) at 13 weeks of HFD. d Quantification of the ratio between bihormonal (insulin- and glucagon positive) cells relative to all α-, β- and bihormonal cells (%) at 8 weeks HFD (**p = 0.0027, *p = 0.0179, *p = 0.0114, n = 4 independent animals, SEM). e Quantification of the ratio between α-cells relative to all α-, β- and bihormonal cells (%) at 8 weeks of HFD. f Quantification of the ratio between β-cells relative to all α-, β- and bihormonal cells (%) at 8 weeks of HFD (*p = 0.0179, *p = 0.0320, *p = 0.0282, n = 4 independent animals, SEM). g Representative immunofluorescence images of islets from WT, PFKFB3^WT DS and PFKFB3^βKO DS mice at 13 weeks of HFD co-immunostained for cleaved caspase-3 (red), insulin (grey), glucagon (green) and nuclei (blue).