Fig. 1: The data acquisition pipeline and acquired results.
From: Multiscale reconstruction of various vessels in the intact murine liver lobe
a The procedure of acquiring dual-wavelength image dataset by high-definition fluorescent micro-optical sectioning tomography (HD-fMOST). b The slice image from the vascular channel of the intact mouse liver dataset. c The slice image from the cytoarchitecture channel of the intact mouse liver dataset. d The zoom-in of the merged channel images indicated in the white boxes in (b) and (c). e, f The zoom-in of the area indicated by the white boxes in (d). The yellow arrows indicate sinusoids. g The zoom-in of the merged channel images indicated in the white boxes in (b). The cyan arrow indicates portal vein. The red arrow indicates hepatic artery. h The zoom-in of the merged channel images indicated with a white box in (g). i Portal triads in vessel channel image. j Portal triads in merged dual-channel image. The blue arrow indicates bile ducts. The projection thickness of (b, c, g) is 100 µm, and the thickness of (d–f, h–j) is 1 µm. Scale bars, b, c 1 mm; d, g–j 50 μm; e, f 20 μm.
