Fig. 3: NJ tree of pollinator wasps based on COI-COII sequences.

Two species, Eupristina verticillata (host fig: F. microcarpa) and Platyscapa sp. (host fig: F. subpisocarpa) were used as outgroups. There were a total of 1844 sites in the final dataset after eliminating gaps and missing data. The ML and BI analyses showed the same tree topology among the clades (Supplementary Figs. 4 and 5). This tree topology also matches that based on nuclear 28 S rDNA and ITS1, except for the grouping of Clade 2 and Clade 3 in the ITS1 tree (see Supplementary Figs. 7 and 8). The numbers at the nodes show NJ and ML bootstrap values and BI posterior probabilities (NJ/ML/BI). The population names of samples are given in parentheses after sample names, and the number after the population name indicates the number of individuals with the same sequences (see Supplementary Data 1). Species names of wasps and their host figs (in parentheses), and population name and number of individuals (in parentheses) are shown for each clade on the right side of the tree. For the wasp pictures, the species name (clade number), individual number (population name) and the scale bars (1 mm) are shown below each picture. For the abbreviations of population names, refer to Fig. 1.