Fig. 5: GSTM2 modulated lipid droplet growth via ASK1 signalling.
From: Glutathione S-transferase Mu 2 inhibits hepatic steatosis via ASK1 suppression
a–c PCYT1A expression level was down-regulated by GSTM2 KD and up-regulated by GSTM2 OE; *p < 0.05, **p < 0.01. d PCYT1A KD induced LD expansion. e, f GSTM2 affected recruitment of GPAT4 and DGAT2 on LD surface (in the presence of oleic acid medium), bar, 10 μm, 1 μm (inlay). g Expression level of GPAT4 and DGAT2 was regulated by treatment of palmitic acid (ASK1 activator) and GS-4997 (ASK1 activity inhibitor). h Recruitment of GPAT4 on LD surface was regulated by palmitic acid or GS-4997 treatment, bar, 10 μm, 1 μm (inlay). i, j qPCR detection of PCYTA1 expression with PA or GS-4997 treatment respectively.
