Fig. 4: Neonatal intestinal macrophages promote endothelial cell sprouting in vitro via IGF-1. | Communications Biology

Fig. 4: Neonatal intestinal macrophages promote endothelial cell sprouting in vitro via IGF-1.

From: Macrophage-derived IGF-1 protects the neonatal intestine against necrotizing enterocolitis by promoting microvascular development

Fig. 4

a, b 4 × 104 mT/mG neonatal intestinal endothelial cells were co-cultured with 2 × 104 CX3CR1+ WT neonatal intestinal macrophages for 4 days on Matrigel in IGF-1-free media (control) or in media containing IGF-1, the IGF-1 inhibitor PPP or both. a Representative images. b Graph represents mean sprout length per well relative to control. Data are the results of three separate experiments combined. n = 6 wells/group, each dot indicates the average length of 5–9 sprouts from a single culture well. P values were calculated using one-way ANOVA followed by Turkey–Kramer multiple-comparison test. **p < 0.01, ****p < 0.0001. Source data are provided as a Source Data file.

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