Fig. 5: Physical and biochemical characterization of SPGR-engineered IgGs.
From: Successive remodeling of IgG glycans using a solid-phase enzymatic platform
a Size analyses using dynamic light scattering (DLS). b Melting temperature analyses (Tm). c Aggregation temperature analyses (Tagg). Endo S treatment removed most IgG glycan structures, leaving only GlcNAc-Fucose di-saccharide on IgG. Box and whiskers graph: median with 25th to 75th percentile. whiskers indicate min and max values (N = 5). d Competition assay revealed the binding affinity of SPGR-engineered IgG to Fc gamma receptor I (FcγR-I). The lower the relative intensity, the stronger the interaction between SPGR-engineered IgG and FcγR-I was. Refer to Fig. 3 and S12 for the chromatograms of analyzed samples.
