Fig. 4: ICAM1 and NCAM1 are candidate targets of miR-27a in U251 and NPC.
From: A functional SNP rs895819 on pre-miR-27a is associated with bipolar disorder by targeting NCAM1
a The relative expression of miR-27a transfected by miR-27a mimics and inhibitor in U251 and NPC were determined by qRT-PCR. The two doses in U251 were 30 pmol and 90 pmol and in NPC were 1 pmol and 10 pmol respectively, with five replicates (n = 5) for every sample. b, c Show the relative protein expression levels of NCAM1 and ICAM1 transfected by miR-27a mimics and inhibitor in U251 and NPC, and its quantification using Compass Simple Western software. Date are representatives with three replicates (n = 3) for each sample. Significant differences between these groups were analyzed using one-way ANOVA, Tukey’s multiple comparisons test for adjusted P values. d, e The relative mRNA expression levels of mature miR-27a, NCAM1, and ICAM1 in miR-27 KO using three different sgRNAs compared to WT. Sg30-mix, sg31-mix, and sg32-mix denote the miR-27 KO in U251, n = 5. f The western blot of NCAM1 and ICAM1 in miR-27 KO using three different sgRNAs and WT, n = 3. g The quantification of NCAM1 and ICAM1 protein levels in U251 cells. Significant differences between these groups were analyzed using one-way ANOVA, and Tukey’s multiple comparisons test were used for adjusted P values. Data were shown as mean ± SD. (*, p < 0.05; **, p < 0.01; ***, p < 0.001; ****, p < 0.0001).
