Fig. 5: NCAM1 is a direct target of miR-27a.
From: A functional SNP rs895819 on pre-miR-27a is associated with bipolar disorder by targeting NCAM1
a The miR-27a binding sites in the 3′UTR of ICAM1, NCAM1. The binding sites were predicted by Targetscan (marked in yellow). The mutated seed sequences of NCAM1-3’UTR and ICAM1-3’UTR were denoted in red. b 293T cells were co-transfected with pmiR-3′UTR-WT/pmiR-3’UTR-M and miR-27 mimic/inhibitor or its NC. Luciferase activity was detected using a dual-luciferase assay system after 48 h. Unpaired t-test was performed between two groups with five replicates (n = 5), * indicates the significant difference compared with NC group (***, p < 0.001; n.s, not significant). Data were shown as mean ± SD.
