Fig. 1: Anatomical localization and vascularization of the rat trigeminal ganglia.
a, e fUS imaging through the whole brain depth reveals the vascularization and localization of the rat TG in a coronal plane (a) or sagittal plane (e). b Capture of the 3D tomographic scan detailed in Supplementary Movie 1, illustrating the TGs seen in 3D (white arrows). c, d Localization of the trigeminal ganglion according to Waxholm’s tractography atlas (https://scalablebrainatlas.incf.org/rat/PLCJB14, i.e., defined using fiber tracks34,35,36), observed coronally (c, white arrows) or in 3D (d, black line surrounding of the ganglia) medio-lateral, at the base of the brain. f, g In toto vascular staining in the rat TG showing that this structure is richly vascularized by a dense network of both thin (10 µm diameter) blood vessels, but also large blood vessels (50 μm diameter). DiI was used to stain the endothelial cell membrane lining the blood vessels, as previously described60. IC internal carotid. Scale bars = a, b, c: 2 mm, e: 1.5 mm, f: 200 μm, g: 100 μm. Images of the Waxholm’s tractography atlas, which are distributed under the Creative Commons Attribute Non-Commercial Share-Alike license, were copied, without any modification.
