Fig. 4: Expression of the adc gene quantified by RT-qPCR in floating colonies and biofilms as a function of pH, urea and ammonia.

For each bacterial lifestyle, conditions within each environmental insult were statistically compared pairwise as illustrated by letters above the corresponding distributions (blue for FFC and orange for SAB). Only letters of the same colours (referring to the same phenotype) can be compared. For each colour, when all letters are different between two distribuitions, then the expression levels in the two corresponding conditions are considered significantly different (Post-Hoc Tukey HSD; p < 0.05). If two distributions share at least one letter, then the expression levels in these conditions are not significantly different with a p ≥ 0.05. a Exposure of P. stuartii to different pHs. b Exposure to increasing concentrations of urea. c Exposure to ammonia.