Table 1 Cryo-EM data collection, refinement and validation statistics.
Adc stack | Adc decamer | |
|---|---|---|
EMDB 13466 PDB 7PK6 | EMDB 13261 PDB 7P9B | |
Data collection and processing | ||
Magnification | 215,000 | 215,000 |
Voltage (kV) | 300 | 300 |
Electron exposure (e−/Å2) | 40.0 | 40.0 |
Defocus range (μm) | −0.5–−2.5 | −0.5–−2.5 |
Pixel size (Å) | 0.65 | 0.65 |
Symmetry imposed | D5 | D5 |
Initial particle images (no.) | 438,429 | 303,475 |
Final particle images (no.) | 268,579 | 46,854 |
Map resolution (Å) | 2.15 | 2.44 |
FSC threshold | 0.143 | 0.143 |
Map resolution range (Å) | 2.07–3.56 | 2.42–3.29 |
Refinement | ||
Initial model used (PDB code) | 2VYC | PDB 7PK6 |
Model resolution (Å) (masked) | 2.42 | 2.56 |
FSC threshold | 0.5 | 0.5 |
Map sharpening B factor (Å2) | −74 Å−2 | −75 Å−2 |
Model composition | ||
Non-hydrogen atoms | 120,580 | 60,290 |
Protein residues | 15,100 | 7550 |
Ligands | 0 | 0 |
B factors (Å−2) | ||
Protein | 23.00 | 47.08 |
R.m.s. deviations | ||
Bond lengths (Å) | 0.006 | 0.009 |
Bond angles (°) | 0.649 | 1.127 |
Validation | ||
MolProbity score | 1.47 | 1.51 |
Clashscore | 4.28 | 7.23 |
Poor rotamers (%) | 2.22 | 0.93 |
Ramachandran plot | ||
Favoured (%) | 98.00 | 97.47 |
Allowed (%) | 2.00 | 2.53 |
Outliers (%) | 0.00 | 0.00 |
