Fig. 1: Podocyte ROCK2 is upregulated in animal models of diabetes and in patients with diabetes.
From: ROCK2-induced metabolic rewiring in diabetic podocytopathy
a ROCK2 levels in the renal cortex of streptozotocin (STZ)-injected mice, db/db mice, and mice treated with high-fat diet (HFD). b The expression levels of ROCK2 in isolated glomeruli. c A correlation analysis between glomerular ROCK2 mRNA and albumin-to-creatinine ratio (ACR). Hodgin Diabetes Mouse Glom data obtained from the transcriptomic database Nephroseq (https://www.nephroseq.com) were used. The trendline is shown in red. The R value was determined by Pearson correlation analysis; white and gray plots represent non-diabetic mice and diabetic mice, respectively (n = 18–21). d Immunostaining of ROCK2 (green) and Nephrin (red) in the glomerulus of STZ-injected mice, db/db mice, and HFD-fed mice. Nuclei were visualized with DAPI (blue). The scale bar on the top left represents 10 μm. e Immunostaining of ROCK2 (green) and Nephrin (red) in the glomeruli of patients without or with diabetes. Nuclei were visualized with DAPI (blue). The scale bar on the top left represents 50 μm. *p < 0.05. Data represent the mean ± s.e.m.
