Fig. 2: Generation of podocyte-specific ROCK2-deficient mice.
From: ROCK2-induced metabolic rewiring in diabetic podocytopathy
a The strategy for the generation of podocyte-specific ROCK2 knockout mice. Gene-targeting vectors were constructed to delete exon 3 of ROCK2. b Representative genotyping PCR detecting transgenic ROCK2-floxed alleles and Podocin-Cre (Pod-Cre). c Confirmation of tissue-restricted-ROCK2 deletion in podocyte-specific ROCK2 knockout mice (PR2KO). The mRNA levels of ROCK2 in the glomerulus (Glom), liver, muscle, and heart are demonstrated (n = 3). d Immunolabeling of ROCK2 and the expression of nephrin in kidney sections obtained from WT and PR2KO mice. The scale bar at the left top represents 10 μm. e Representative images of the whole body (upper panel) and kidney (lower panel) in each genotype. The scale bar on the top left represents 1 cm. f Representative PAS staining and transmission electron microscope (TEM) images of the glomerulus from WT and PR2KO mice. The scale bars at the top represents 10 μm and 1 μm, respectively. g Body weight information in aged WT and PR2KO (n = 9–11). h ACR at 12 months of age (n = 9–10). *p < 0.05. Data represent the mean ± s.e.m.
