Fig. 3: Ablation of podocyte ROCK2 prevents diabetic renal damage in mice.
From: ROCK2-induced metabolic rewiring in diabetic podocytopathy
a The breeding scheme that was used to generate PR2KO mice. Diabetes was induced by STZ injection, mating with db/m mice to generate db/db mice, or HFD feeding. b ACR in STZ-injected, db/db, HFD-fed WT and PR2KO mice (n = 4–8). c The kidney weight in the three experimental groups (n = 4–8). d Representative PAS-stained images of kidney glomeruli from mice. The scale bar on the top left represents 10 μm (n = 4–6). e Wilms tumor 1 (WT1) immunostaining and quantification of WT1-positive cells in glomeruli from mice. The scale bar on the top left represents 10 μm (n = 4). f Foot process width examined by transmission electron microscopy. The scale bar on the top left represents 1 μm (n = 4). g Glomerular basement membrane (GBM) thickness assessed by transmission electron microscopy. The scale bar on the top left represents 0.5 μm (n = 4). *p < 0.05. Data represent the mean ± s.e.m.
